http://mafft.cbrc.jp/alignment/software/
MAFFT is a multiple sequence alignment program for unix-like operating systems. It offers a range of multiple alignment methods, L-INS-i (accurate; for alignment of <∼200 sequences), FFT-NS-2 (fast; for alignment of <∼10,000 sequences), etc. 200条以内序列的精确比对,10,000条序列的快速比对,呵呵,序列比对的数量这个比其他软件有优势哈哈。http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923890/